Publication Database

Feeding of Enterococcus faecium NCIMB 10415 leads to intestinal miRNA-423-5p induced regulation of immune-relevant genes (2016)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Kreuzer-Redmer, Susanne

Bekurtz, Jennifer C

Arends, Danny

Bortfeldt, Ralf

Kutz-Lohroff, Barbara (WE 3)

Sharbati, Soroush (WE 3)

Einspanier, Ralf (WE 3)

Brockmann, Gudrun A

Quelle

Applied and environmental microbiology

Bandzählung: 82

Heftzählung: 8

Seiten: 2263 – 2269

ISSN: 1098-5336

Sprache

Englisch

Verweise

DOI: 10.1128/AEM.04044-15

Pubmed: 26826223

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Probiotics are widely used in human and animal health, but little is known about the mode of action of probiotics. One possible mechanism on a molecular level could be an influence on miRNAs and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissue from Enterococcus faecium NCIMB 10415-fed piglets versus untreated controls using Next Generation Sequencing. We identified the miR-423-5p as highly affected by the treatment group (2.32 fold; P = 0.014). Validation by RT-qPCR confirmed a significant up-regulation of miR-423-5p (2.11 fold; P = 0.03) and, additionally a down-regulation of important immune-relevant target gene IGLC (0.61 fold; P = 0.03) and IGKC (0.69 fold; P = 0.04). Expression analysis of miR-423-5p and IGLC at different age points, show a clear anti-correlated relation. Luciferase reporter assays in a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding E. faecium on the expression of miR-423-5p and on the regulation of the immune globulin gene IGLC through miR-423-5p. This might be a possible mode of action of E. faecium on immune cell regulation in the small intestine.