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The overall objective of this study was to validate an electronic nose device for differentiating milk samples from dairy cows with and without mastitis.
In experiment 1 the influence of volume, freezing, boric acid and originating farm was evaluated. While there was no effect of volume, frozen samples and samples with and without boric acid could be distinguished. Sensitivity and specificity were highest (i.e., 90 %) for milk samples from different farms.
In experiment 2 a differentiation between healthy and mastitis samples was not reliable. The differentiation between gram-positive and negative pathogens, as well as between Staphylococcus aureus and other gram-positive pathogens was moderate. There seems to be potential for mastitis detection using an electronic nose device but further research is necessary.