Fachbereich Veterinärmedizin



    Arcobacter butzleri isolates exhibit pathogenic potential in intestinal epithelial cell models (2015)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Karadas, Gül (WE 8)
    Bücker, Roland
    Sharbati, Soroush (WE 3)
    Schulzke, Jörg-Dieter
    Alter, Thomas (WE 8)
    Gölz, Greta (WE 8)
    Journal of Applied Microbiology; 120(1) — S. 218–225
    ISSN: 1364-5072
    DOI: 10.1111/jam.12979
    Pubmed: 26481610
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
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    Abstract / Zusammenfassung

    The pathogenic potential of Arcobacter (A.) butzleri isolates on human (HT-29/B6) and porcine epithelial (IPEC-J2) cells was investigated by in vitro assays.

    Five out of six A. butzleri isolates were able to adhere and invade HT-29/B6 cells while only four isolates adhered and two invaded IPEC-J2 cells. Two non- or poorly invasive A. butzleri isolates were highly cytotoxic to differentiated HT-29/B6 cells but none to IPEC-J2 cells as determined by WST-assays. Epithelial integrity of cell monolayers, monitored by measurement of the transepithelial electrical resistance (TER), was decreased by all A. butzleri isolates in HT-29/B6 and IPEC-J2 cells to 30 -15 % and 90 - 50%, respectively.

    The A. butzleri strain-specific pathomechanisms observed with the human colon cell line HT-29/B6, like adhesion, invasion and cytotoxicity might all contribute to epithelial barrier dysfunction, which could explain a leak-flux type of diarrhea in humans. In contrast, porcine cells seem to be less susceptible to A. butzleri.

    A. butzleri has enteric pathogenic potential, characterized by defined interactions with human epithelial cells and strain-specific pathomechanisms. This article is protected by copyright. All rights reserved.