Fachbereich Veterinärmedizin



    Arcobacter butzleri induce inflammatory responses in gnotobiotic IL-10 deficient mice (2015)

    Gölz, G. (WE 8)
    Karadas, G. (WE 8)
    Alter, T. (WE 8)
    Bereswill, S. (WE 10)
    Heimesaat, M.M. (WE 10)
    National Symposium on Zoonoses Research
    Berlin, 15. – 16.10.2015
    National Symposium on Zoonoses Research : 15 - 16 October 2015 ; Program and Abstracts — German Research Platform for Zoonoses (Hrsg.)
    Berlin, 2015 — S. 145
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
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    Abstract / Zusammenfassung

    Background and objectives: Acute gastroenteritis with abdominal pain and acute or prolonged watery diarrhoea has been described for humans infected with Arcobacter(A.) butzleri. Adhesive, invasive and cytotoxic capacities have been described for A. butzleri in vitro. So far, only limited information is available about the immunepathogenic mechanisms of infection in vivo.
    The aim of this study was to investigate the immune-pathological properties of A. butzleri in a well-established murine infection model.
    Methods: Gnotobiotic IL-10-/- mice were orally infected with two A. butzleri strains and clinical signs as well as fecal shedding were determined over time. At day 6 and day 16 post·infection apoptotic and proliferating cells, intestinal infiltration with immune cells and cytokine expression patterns were determined.
    Results: Despite no overt macroscopic signs of disease, stable infection of gnotobiotic IL-10 -/- mice with A. butzleri led to increased numbers of apoptotic cells, influx of immune cells and higher expression of pro-Inflammatory cytokines in the intestine, depending on the respective A. butzleri strain.
    Conclusion: Even though no overt clinical signs have been observed we could clearly show that A. butzleri is able to stably colonize and induce apoptosis paralleled by induction of pro-inflammatory immune responses in the intestine of infected gnotobiotic IL-10-/- mice, pointing towards an immune-pathogenic potential of A. butzleri in vivo.