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    Epidemiological relationship of ESBL-/ AmpC-producing Enterobacteriaceae in the broiler production chain (2015)

    Art
    Vortrag
    Autoren
    Projahn, M. (WE 10)
    Dähre, K. (WE 10)
    von Tippelskirch, P. (WE 8)
    Orquera, S. (WE 8)
    Alter, T. (WE 8)
    Friese, A. (WE 10)
    Rösler, U. (WE 10)
    Forschungsprojekt
    Verbund RESET II: Verbreitung von ESBL-/AmpC-bildenden Enterobakterien entlang der gesamten Masthähnchenkette: Schwachstellenanalyse und Bestimmung von Interventionspunkten
    Kongress
    National Symposium on Zoonoses Research
    Berlin, 15. – 16.10.2015
    Quelle
    National Symposium on Zoonoses Research : 15 - 16 October 2015 ; Program and Abstracts — German Research Platform for Zoonoses (Hrsg.)
    Berlin, 2015 — S. 57
    Sprache
    Englisch
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    +49 30 838 62550
    lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Background and objectives: Enterobacteriaceae producing extendedspectrum beta-lactamases (ESBL) or AmpC beta-lactamases are occurring in many herds on broiler farms in Germany. Little is known about the origin of these resistant bacteria in fattening farms and the vertical and horizontal transfer of ESBL-/AmpC producing strains along the broiler production chain. In our study we, therefore, investigate for the first time batch depending transmission routes in the entire broiler production chain.
    Materials and methods: We collect faecal and environmental samples from seven parent flocks, their eggs and the environment in the hatchery and take samples from fattening farms and final ly the slaughter house tracking each flock separately. Suspicious enterobacteria were investigated concerning their phylogenetic groups and ESBL/AmpC genes to determine first potential epidemiological relationships.
    Results: Escherichia coli isolates originating from the first analysed production chain were assigned to Phylogroup A harbouring a TEM-52 gene (parent flock), to Phylogroup A, B1, F or E/D which were positive tested for CMY-2 genes (fattening farm) and Phylogroup A, B1, E, E/D harbouring gene combinations of CMY-2, TEM-52 and CTX-M15 (slaughterhouse).
    Conclusion: First comparisons of ESBL-/AmpC-Genes and phylogenetic groups of isolates show that an exclusively vertical transfer of ESBL-/AmpC producing enterobacteria seems to be not very likely but further investigations will be done.