Publikationsdatenbank

Identification of novel splice variants of the voltage- and Ca²⁺-dependent K⁺-channel SLO-1 of Trichuris muris (2015)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Yilmaz, Esra (WE 13)

Kulke, Daniel

von Samson-Himmelstjerna, Georg (WE 13)

Krücken, Jürgen (WE 13)

Quelle

Molecular and biochemical parasitology

Bandzählung: 199

Heftzählung: 1-2

Seiten: 5 – 8

ISSN: 0166-6851

Sprache

Englisch

Verweise

DOI: 10.1016/j.molbiopara.2015.03.001

Pubmed: 25779980

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The anthelmintic cyclooctadepsipeptide emodepside is effective against nematodes showing resistance against established drug classes. Emodepside exerts its nematicidal effects mainly through its validated target, the tetrameric voltage- and calcium-activated potassium channel SLO-1. Two slo-1 genes were described in Trichuris muris. Alternative splicing is known to alter SLO-1 properties. Here, 16 T. muris splice variants for slo-1.1 and three variants for slo-1.2 were identified in addition to previously described variants. Splice variants caused by intron retentions and/or exon exclusions encode varyingly truncated subunits. Depending on the subunit composition, channels might have altered physiological and pharmacological properties including different modulation by calcium and/or voltage or reduced emodepside susceptibility which might lead to emodepside resistance as observed in Caenorhabditis elegans expressing only similarly truncated Slo-1. The comprehensive characterisation of splice variants is a prerequisite for functional analysis and confirmed conservation of remarkable differences found between both slo-1 paralogs in Trichuris suis.