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    Human microvascular endothelial cells displaying reduced angiogenesis and increased uptake of lipids during in vitro culture (2015)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Sievers, Henrieke (WE 1)
    Hirschberg, Ruth M
    Hiebl, Bernhard
    Hünigen, Hana (WE 1)
    Plendl, Johanna (WE 1)
    Quelle
    Clinical hemorheology and microcirculation; 61(2) — S. 367–383
    ISSN: 1386-0291
    Verweise
    DOI: 10.3233/CH-152002
    Pubmed: 26444614
    Kontakt
    Institut für Veterinär-Anatomie

    Koserstr. 20
    14195 Berlin
    +49 30 838 53555
    anatomie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Human microvascular ECs from the neonatal foreskin of two donors purchased from one distributor were used in an angiogenesis assay under the same culture conditions. Different angiogenic potency was apparent in these two batches (ECang and ECnon-ang). During the cultivation period of three weeks, ECang ran through all stages of angiogenesis starting from proliferation to migration up to the formation of three-dimensional capillary-like structures. Despite of expression of endothelial markers, ECnon-ang showed excessive intracellular storage of lipids in form of multilamellar bodies and decreased angiogenic potency in contrast to its counterpart, ECang. Results indicate that lipid metabolism differs in ECang versus ECnon-ang. This study points up that these differences are based on the different donors and presents a novel and valuable model for the study of mechanisms of atherosclerosis in endothelial cells in vitro.