Fachbereich Veterinärmedizin



    Thermoregulation in a 5-HT 1A receptor mutant mouse line (2015)

    Dietze, Silke (WE 14)
    Klein, Laura (WE 14)
    Brosda, Jan (WE 14)
    Fink, Heidrun (WE 14)
    81st Annual Congress of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT)
    Kiel, 10. – 12.03.2015
    Naunyn-Schmiedeberg´s Archives of Pharmacology; 388(Suppl.1) — S. 29
    ISSN: 0028-1298
    URL (Volltext): http://link.springer.com/article/10.1007%2Fs00210-015-1087-4
    DOI: 10.1007/s00210-015-1087-4
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    Abstract / Zusammenfassung

    The serotonin 1A (5-HT1A) -receptor is involved in a wide range of physiological functions such as in the regulation of body temperature. Studies have shown that in mice, the hypothermic response induced by the full agonist at the 5-HT1A-receptor 8-OH-DPAT is mediated by presynaptic 5-HT1A-receptors. In contrast, investigations in humans and rats detected an involvement of postsynaptic 5-HT1A-receptors. In our study we used a transgenic mouse line with a permanent overexpression of the 5-HT1A-receptor in the projection areas of serotonergic neurons. We investigated the basal body temperature and the hypothermic effect of different dosages of 8-OH-DPAT (0.1mg/kg – 4 mg/kg ip.) in male transgenic mice in comparison to NMRI wild-type males using radio telemetry, a method that allows non-invasive recordings of body temperature. The basal body temperature of transgenic mice was lower than in NMRI wild-type mice (transgenic mice: 36.0 °C; NMRI wild-type mice: 37.4 °C). In both genotypes, hypothermia by systemic administration of 8-OH-DPAT was induced in a dose dependent manner. However, the temperature decrease was more pronounced in transgenic mice with -2.8 °C compared to -1.5 °C in NMRI wild-types. Dose response curves of 8-OH-DPAT revealed an ED50 = 0.4 mg/kg in transgenic and ED50 = 0.57 mg/kg in NMRI wild-type mice. Our results suggest that the postsynaptic 5-HT1A-receptor is involved in the regulation of body temperature in mice.