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    Adhesion of Trypanosoma congolense activates signalling pathways in endothelial cells. (2004)

    Art
    Poster
    Autoren
    Bondzio, A.
    Reinwald, E.
    Kongress
    Herbsttagung der Gesellschaft für Biochemie und Molekularbiologie
    Münster, 19. – 22.09.2004
    Quelle
    Sprache
    Englisch
    Kontakt
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62225
    biochemie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Contrary to other African trypanosomes Trypanosoma congolense bloodstream forms
    specifically adhere to endothelial cells. Nothing is known about the receptors involved
    and the biological significance of this phenomen. In this study we show that signal
    transduction pathways are activated in human umbilical vein endothelial cells (HUVECs)
    during trypanosomes adhesion.
    Adhesion between Trypanosoma congolense and HUVECs is only transient, with a
    maximum effect at 2 hours incubation time. After that the adhesion is declined. In
    parallel to the increase and decrease of adhesion NF-kB is activated and IL-8 is secreted
    into the medium. In addition, HUVECs produce reactive oxygen species (ROS) during
    adhesion, as measured with pholasin. Preincubation of HUVECs with the antioxidants Nacetyl-
    L-cysteine (NAC) and pyrrolidine dithiocarbamate (PDTC) and the NAD(P)H
    oxidase inhibitor apocynin completely abolish NF-kB activation, IL-8 secretion and
    Trypanosoma congolense adhesion. Thus, either ROS or NF-kB or both may be involved
    in the regulation of the activity of the adhesion receptor.