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    Estimation of most probable number of Salmonella in retail samples of minced pork (1990)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Sinell, Hans-Jürgen (WE 8)
    Pietzsch, Otfried
    Klingbeil, Harry (WE 8)
    Benner, Michael
    Quelle
    International Journal of Food Microbiology; 11(2) — S. 135–141
    ISSN: 0168-1605
    Sprache
    Englisch
    Verweise
    Pubmed: 2261315
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    +49 30 838 62550
    lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The number of salmonellae in 174 samples of minced pork was determined by the Most Probable Number (MPN) method in two separate laboratories. The material examined was taken from a collection of samples which were Salmonella positive in an earlier study. The MPN estimation was carried out using portions of original samples which had been divided (2 x 100 g) before the initial examination and which had been deep frozen and stored for 1 to 14 weeks at -18 degrees C until re-examination. Of the 174 samples initially positive for Salmonella, 131 (75.3%) were positive on re-examination using pre-enrichment in buffered peptone water (BPW) and selective enrichment in Rappaport-Vassiliadis medium (RV) and in tetrathionate medium according to Muller-Kauffmann (MK). The majority of the samples gave Salmonella counts below 1000/100 g (96.7% at lab. A and 97.3% at lab. B). Comparison of the results from both laboratories showed good agreement in the distribution pattern of the frequencies within the MPN classes, but agreement between the same sample pair was poor (r = 0.23). RV medium proved to be superior to the MK medium.