Fachbereich Veterinärmedizin



    Cyclic-di-GMP signalling and biofilm-related properties of the Shiga toxin-producing 2011 German outbreak Escherichia coli O104:H4 (2014)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Richter, Anja M
    Povolotsky, Tatyana L
    Wieler, Lothar H (WE 7)
    Hengge, Regine
    EMBO molecular medicine; 6(12) — S. 1622–1637
    ISSN: 1757-4676
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000021574
    DOI: 10.15252/emmm.201404309
    Pubmed: 25361688
    Institut für Mikrobiologie und Tierseuchen

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    14163 Berlin
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    Abstract / Zusammenfassung

    In 2011, nearly 4,000 people in Germany were infected by Shiga toxin (Stx)-producing Escherichia coli O104:H4 with > 22% of patients developing haemolytic uraemic syndrome (HUS). Genome sequencing showed the outbreak strain to be related to enteroaggregative E. coli (EAEC), suggesting its high virulence results from EAEC-typical strong adherence and biofilm formation combined to Stx production. Here, we report that the outbreak strain contains a novel diguanylate cyclase (DgcX)--producing the biofilm-promoting second messenger c-di-GMP--that shows higher expression than any other known E. coli diguanylate cyclase. Unlike closely related E. coli, the outbreak strain expresses the c-di-GMP-controlled biofilm regulator CsgD and amyloid curli fibres at 37°C, but is cellulose-negative. Moreover, it constantly generates derivatives with further increased and deregulated production of CsgD and curli. Since curli fibres are strongly proinflammatory, with cellulose counteracting this effect, high c-di-GMP and curli production by the outbreak O104:H4 strain may enhance not only adherence but may also contribute to inflammation, thereby facilitating entry of Stx into the bloodstream and to the kidneys where Stx causes HUS.