Publikationsdatenbank

Effect of incubation temperature on the diagnostic sensitivity of the glanders complement fixation test (2014)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Khan, I

Wieler, L H (WE 7)

Saqib, M

Melzer, F

Santana, V L D A

Neubauer, H

Elschner, M C

Quelle

Revue scientifique et technique; 33(3) — S. 869–875

ISSN: 0253-1933

Sprache

Englisch

Verweise

Pubmed: 25812210

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
Gebäude 35
14163 Berlin
+49 30 838 51840 / 51843
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The complement fixation test (CFT) is the only serological test prescribed by the World Organisation for Animal Health (OIE) for the diagnosis of glanders in international trading of equids. However, false-positive reactions have caused financial losses to the animal owners in the past, and false-negative tests have resulted in the introduction of glanders into healthy equine populations in previously glanders-free areas. Both warm (incubation at 37°C for 1 h) and cold (overnight incubation at 4°C) procedures are recommended by the OIE for serodiagnosis of glanders. In a comparison of the sensitivity and specificity of the two techniques, using the United States Department of Agriculture antigen, warm CFT was found to be significantly less sensitive (56.8%; p < 0.0005) than the cold CFT (83.6%). Cold CFT thus increases the detection rate of glanders but a lower diagnostic specificity has to be accepted. The immunoblot was used as the gold standard.