Fachbereich Veterinärmedizin



    Quantification of MDR-1 Gene Expression in Canine Tissues by Real-time Reverse Transcription Quantitative Polymerase Chain Reaction (2004)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Culmsee, K
    Gruber, A D (WE 12)
    von Samson-Himmelstjerna, G
    Nolte, I
    Research in Veterinary Science; 77(3) — S. 223–229
    ISSN: 0034-5288
    Pubmed: 15276773
    Institut für Tierpathologie

    Robert-von-Ostertag-Str. 15
    Gebäude 12
    14163 Berlin
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    Abstract / Zusammenfassung

    MDR-1 gene product mediated multidrug resistance is thought to play a major role in the outcome of chemotherapy in some canine tumors, especially malignant lymphoma. In the present study, MDR-1 RNA expression in normal lymph node and liver tissue as well as in tumor biopsies from 23 dogs with lymphomas and two dogs with liver tumors was measured by real-time RT-quantitative PCR. MDR-1 gene expression was detected in all samples analyzed. Comparably high MDR-1 RNA levels were measured in all normal liver tissues, one of the lymphomas and a cholangiocarcinoma. MDR-1 expression levels in canine lymphomas were found to vary over a wide range with most tumors expressing relative low levels. Interestingly, gastrointestinal lymphomas expressed higher MDR-1 RNA levels than multicentric lymphomas (p = 0.03). In conclusion, real-time RT-quantitative PCR appears to be a suitable method for sensitive and quantitative determination of MDR-1 gene expression in canine normal and neoplastic tissues.