Fachbereich Veterinärmedizin



    Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter seasons (2015)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Rödiger, Stefan
    Kramer, Toni
    Frömmel, Ulrike
    Weinreich, Jörg
    Roggenbuck, Dirk
    Guenther, Sebastian (WE 7)
    Schaufler, Katharina (WE 7)
    Schröder, Christian
    Schierack, Peter
    Environmental microbiology; 17(9) — S. 3352–3361
    ISSN: 1462-2912
    DOI: 10.1111/1462-2920.12807
    Pubmed: 25684458
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51840 / 51843

    Abstract / Zusammenfassung

    We report the population structure and dynamics of one Escherichia coli population of wild mallard ducks in their natural environment over four winter seasons, following the characterization of 100 isolates each consecutive season. Macro-restriction analysis was used to define isolates variously as multi- or 1-year pulsed-field gel electrophoresis (PFGE) types. Isolates were characterized genotypically based on virulence-associated genes (VAGs), phylogenetic markers, and phenotypically based on haemolytic activity, antimicrobial resistance, adhesion to epithelial cells, microcin production, motility and carbohydrate metabolism. Only 12 out of 220 PFGE types were detectable over more than one winter, and classified as multi-year PFGE types. There was a dramatic change of PFGE types within two winter seasons. Nevertheless, the genetic pool (VAGs) and antimicrobial resistance pattern remained remarkably stable. The high diversity and dynamics of this E. coli population were also demonstrated by the occurrence of PFGE subtypes and differences between isolates of one PFGE type (based on VAGs, antimicrobial resistance and adhesion rates). Multi- and 1-year PFGE types differed in antimicrobial resistance, VAGs and adhesion. Other parameters were not prominent colonization factors. In conclusion, the high diversity, dynamics and stable genetic pool of an E. coli population seem to enable their successful colonization of host animal population over time.