Fachbereich Veterinärmedizin



    Species-wide whole genome sequencing reveals historical global spread and recent local persistence in Shigella flexneri (2015)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Connor, Thomas R
    Barker, Clare R
    Baker, Kate S
    Weill, François-Xavier
    Talukder, Kaisar Ali
    Smith, Anthony M
    Baker, Stephen
    Gouali, Malika
    Pham Thanh, Duy
    Jahan Azmi, Ishrat
    Dias da Silveira, Wanderley
    Semmler, Torsten (WE 7)
    Wieler, Lothar H (WE 7)
    Jenkins, Claire
    Cravioto, Alejandro
    Faruque, Shah M
    Parkhill, Julian
    Wook Kim, Dong
    Keddy, Karen H
    Thomson, Nicholas R
    eLife; 2015(4) — S. e07335
    ISSN: 2050-084x
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000023040
    DOI: 10.7554/eLife.07335
    Pubmed: 26238191
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    Abstract / Zusammenfassung

    Shigella flexneri is the most common cause of bacterial dysentery in low-income countries. Despite this, S. flexneri remains largely unexplored from a genomic standpoint and is still described using a vocabulary based on serotyping reactions developed over half-a-century ago. Here we combine whole genome sequencing with geographical and temporal data to examine the natural history of the species. Our analysis subdivides S. flexneri into seven phylogenetic groups (PGs); each containing two-or-more serotypes and characterised by distinct virulence gene complement and geographic range. Within the S. flexneri PGs we identify geographically restricted sub-lineages that appear to have persistently colonised regions for many decades to over 100 years. Although we found abundant evidence of antimicrobial resistance (AMR) determinant acquisition, our dataset shows no evidence of subsequent intercontinental spread of antimicrobial resistant strains. The pattern of colonisation and AMR gene acquisition suggest that S. flexneri has a distinct life-cycle involving local persistence.