Publikationsdatenbank

Species-wide whole genome sequencing reveals historical global spread and recent local persistence in Shigella flexneri (2015)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Connor, Thomas R

Barker, Clare R

Baker, Kate S

Weill, François-Xavier

Talukder, Kaisar Ali

Smith, Anthony M

Baker, Stephen

Gouali, Malika

Pham Thanh, Duy

Jahan Azmi, Ishrat

Dias da Silveira, Wanderley

Semmler, Torsten (WE 7)

Wieler, Lothar H (WE 7)

Jenkins, Claire

Cravioto, Alejandro

Faruque, Shah M

Parkhill, Julian

Wook Kim, Dong

Keddy, Karen H

Thomson, Nicholas R

Quelle

eLife; 2015(4) — S. e07335

ISSN: 2050-084x

Sprache

Englisch

Verweise

URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000023040

DOI: 10.7554/eLife.07335

Pubmed: 26238191

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
Gebäude 35
14163 Berlin
+49 30 838 51840 / 51843
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Shigella flexneri is the most common cause of bacterial dysentery in low-income countries. Despite this, S. flexneri remains largely unexplored from a genomic standpoint and is still described using a vocabulary based on serotyping reactions developed over half-a-century ago. Here we combine whole genome sequencing with geographical and temporal data to examine the natural history of the species. Our analysis subdivides S. flexneri into seven phylogenetic groups (PGs); each containing two-or-more serotypes and characterised by distinct virulence gene complement and geographic range. Within the S. flexneri PGs we identify geographically restricted sub-lineages that appear to have persistently colonised regions for many decades to over 100 years. Although we found abundant evidence of antimicrobial resistance (AMR) determinant acquisition, our dataset shows no evidence of subsequent intercontinental spread of antimicrobial resistant strains. The pattern of colonisation and AMR gene acquisition suggest that S. flexneri has a distinct life-cycle involving local persistence.