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tierhygiene@vetmed.fu-berlin.de
Introduction
Previous studies showed the occurrence of ESBL-/AmpC-producing Enterobacteriaceae in different farm animals. Up
to the present these resistant bacteria were not investigated systematically in turkeys. This cross-sectional study is to
ascertain the prevalence of ESBL-/AmpC-producing Enterobacteriaceae in a representative survey distributed over
Germany.
Materials and Methods
In this study 48 turkey farms are investigated, which are selected depending on the turkey population in each federal
state in Germany. Pooled feces, dust samples and boot swabs are parallel collected in two different age groups. On
the one hand these samples are taken in the rearing unit of a farm, on the other hand in the fattening unit.
In most of the samples ESBL-/AmpC-producing Enterobacteriaceae are detected qualitatively after enrichment and
also quantitatively. MacConkey-Agar with 1μg/ml Cefotaxime is used for selection. All ESBL-/AmpC-suspected microorganisms
are confirmed by disc-diffusion-assay and detecting ESBL- (CTX-M, TEM, SHV) and AmpC-genes
(CMY).
Results
So far 38 of 48 turkey farms are evaluated statistically. In nearly 60% of the investigated farms a positive ESBL-
/AmpC-status could be detected, which means that at least one of the ten samples within one farm was ESBL-
/AmpC-positive. Regarding all samples approximately one third of the samples were positive for ESBL-/AmpCproducing
Enterobacteriaceae.
There is no significant difference between the prevalence in samples originating from the rearing units and the fattening
units.
Conclusions
This study is to collect data of ESBL-/AmpC-producing Enterobacteriaceae in fattening turkey farms in Germany. In
comparison to similar previous studies in poultry, especially broiler, the prevalence of ESBL-/AmpC-producing Enterobacteriaceae
is lower and the predominant ESBL-/AmpC-gene was CTX-M.