Fachbereich Veterinärmedizin



    Transmission of ESBL-/AMPC-producing enterobacteriaceae along the entire production chain of broilers (2015)

    Dähre, Katrin (WE 10)
    Projahn, Michaela (WE 10)
    von Tippelskirch, P. (WE 8)
    Orquera, S. (WE 8)
    Alter, T. (WE 8)
    Friese, Anika (WE 10)
    Rösler, Uwe (WE 10)
    XVII ISAH Congress 2015
    Košice, Slovakia, 07. – 11.06.2015
    Proceeding of the XVII International Congress of the International Society for Animal Hygiene “Animal hygiene and welfare in livestock production – the first step to food hygiene” — University of Veterinary Medicine and Pharmacy in Košice (Hrsg.)
    Košice, Slovakia: Edition centrum and specialised literature shop of University of Veterinary Medicine and Pharmacy in Košice, 2015 — S. 92
    ISBN: 978-80-8077-462-2
    URL (Volltext): http://www.isah-soc.org/userfiles/downloads/proceedings/2015_Proceedings.pdf
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845

    Abstract / Zusammenfassung

    The occurrence of extended-spectrum ß-lactamase (ESBL) and/or plasmid-mediated AmpC ß-lactamase- producing
    Enterobacteriaceae in broiler fattening farms was shown in previous studies. The detection level of these antibiotic
    resistant bacteria was already high in cloacal swabs of one-day old chicken. This leads to the hypothesis of an early
    entry or emergence of these resistant bacteria within the broiler production chain, maybe with a strong impact of the
    hatcheries’ management or the parent flocks.
    Material and Methods
    We are investigating this hypothesis by tracking seven broiler flocks along the entire production chain starting from
    the parent flocks. Several samples originating from the eggs/animals as well as from their direct environment in the
    hatchery/barn are collected. First, the presence of ESBL-/AmpC-producing Enterobacteriaceae is investigated in the
    parent flocks, only positive flocks are included in this study. The hatching eggs of the particular parent flocks are
    traced at three different time points and at specific locations of hazard inside the hatchery. After that the chicken from
    the same batch of hatched eggs as investigated before will be analysed three times during their fattening period in
    the farm, from arrival to the end. Then the same flocks are sampled at the slaughterhouse.
    The analysis of the first broiler chain showed no findings of ESBL-/AmpC-producing Enterobacteriaceae within the
    hatchery. Here the resistant bacteria initially appeared in the middle of the fattening period. In contrast to this in the
    second investigated chain ESBL-/AmpC-producing Enterobacteriaceae were already found in the hatchery.
    By now a precise conclusion about the transmission of the resistant bacteria along the broiler production chain cannot
    be given. More production chains will be investigated and further analyses will lead to information about molecular
    characteristics and relationships of different isolates. These results of the ongoing project will be presented.