Fachbereich Veterinärmedizin



    Occurrence of decreased disinfectant susceptibility among ESBL- / AMPC-producing E. Coli isolated from broilers (2015)

    Li, Taiyun (WE 10)
    Roschanski, Nicole (WE 10)
    Friese, Anika (WE 10)
    Rösler, Uwe (WE 10)
    XVII ISAH Congress 2015
    Košice, Slovakia, 07. – 11.06.2015
    Proceeding of the XVII International Congress of the International Society for Animal Hygiene “Animal hygiene and welfare in livestock production – the first step to food hygiene” — University of Veterinary Medicine and Pharmacy in Košice (Hrsg.)
    Košice, Slovakia: Edition centrum and specialised literature shop of University of Veterinary Medicine and Pharmacy in Košice, 2015 — S. 91
    ISBN: 978-80-8077-462-2
    URL (Volltext): http://www.isah-soc.org/userfiles/downloads/proceedings/2015_Proceedings.pdf
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845

    Abstract / Zusammenfassung

    Disinfectants are widely used in different areas and an inappropriate use might cause the emergence of disinfectantresistances,
    leading to further disinfection failure. Beyond this, the co-selection of disinfectant- and antibiotic- resistance
    has been reported in case of various bacteria. Due to the fact that the presence of ESBL/ AmpC dramatically
    limits the therapeutic options, the appearance of disinfectant-resistances in these kinds of bacteria might contribute a
    health thread for humans as well as animals.
    Materials and Methods
    90 ESBL-/ AmpC-producing E. coli, isolated from cloacal swabs of chicken out of seven broiler fattening flocks
    throughout Germany were investigated. For each strain the minimal inhibitory concentration (MIC) of three different
    disinfectants – Benzalkonium chloride (BKC), Chlorhexidine (CHX) and Triclosan (TCL) was determined. In addition,
    the presence of 5 different qac genes (qacE, qacEΔ1,qacH, qacF and qacG) was investigated.
    Compared to three E. coli reference strains, 23 samples showed decreased susceptibility to single or multiple disinfectants
    (8/CHX; 7/TCL; 3/BKC+CHX; 5/CHX+TCL). However, none of the strains was able to survive at concentrations
    recommended for disinfection with commercially available products.
    The qacEΔ1 gene was found in 18.89% samples, the frequency of qacH 6.67% was low while qacE, qacF and qacG
    were not detected. However, all isolates showing an increased MIC against BKC contain none of the investigated
    qac genes, whereas three BKC sensitive isolates contain qacH as well as qacEΔ1 genes.
    ESBL-/ AmpC- producing E. coli possessing decreased susceptibility to disinfectants have been detected within
    chicken in German broiler fatting farms. However, none of the detected MIC values indicate that accurately performed
    disinfection procedures will lead to ineffectiveness. In addition, no correlation between the presence of qac
    genes and the increased disinfectant tolerance has been observed.