Fachbereich Veterinärmedizin



    Emission of ESBL/AMPC-producing E. Coli from broiler and turkey fattening farms in Germany (2015)

    Lu, Helene Morgenstern (WE 10)
    Laube, Henriette (WE 10)
    Käsbohrer, Annemarie
    Friese, Anika (WE 10)
    Rösler, Uwe (WE 10)
    XVII ISAH Congress 2015
    Košice, Slovakia, 07. – 11.06.2015
    Proceeding of the XVII International Congress of the International Society for Animal Hygiene “Animal hygiene and welfare in livestock production – the first step to food hygiene” — University of Veterinary Medicine and Pharmacy in Košice (Hrsg.)
    Košice, Slovakia: Edition centrum and specialised literature shop of University of Veterinary Medicine and Pharmacy in Košice, 2015 — S. 90
    ISBN: 978-80-8077-462-2
    URL (Volltext): http://www.isah-soc.org/documents/2015/XVII_ISAH_CONGRESS_2015_Proceedings.pdf
    Institut für Tier- und Umwelthygiene

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    Abstract / Zusammenfassung

    Extended-spectrum beta-lactamases (ESBL) reduce dramatically the effectiveness of modern extended-spectrum
    cephalosporins and monobactams. The aim of this study was to comparing investigate the occurrence of these microorganisms
    in the air and on soil surfaces close to ESBL/AmpC positive broiler and turkey fattening farms including
    the possible spread of these bacteria via the faecal rout and/or via exhaust stable air in the environment.
    Materials and Methods
    We investigated seven German broiler and turkey fattening farms, each, three times during one fattening period.
    Therefore, slurry samples, exhaust air samples and various surfaces in the vicinity of the barns were investigated.
    Additionally various samples inside the barns including samples of animals, environmental samples and air samples
    were taken in parallel. For phenotypic detection of ESBL/AmpC-producing E. coli all taken samples were cultivated
    on MacConkey-agar with 1μg/ml Cefotaxime. The occurrence of different ESBL/AmpC-genes was confirmed by PCR
    and if necessary by sequencing.
    Preliminary results show a wide spread of ESBL/AmpC-producing E. coli in pig and broiler farms as well as an emission
    in their surroundings. All slurry samples originating from broilers were positive for ESBL/AmpC-producing E. coli.
    Additionally 28.8 % of all boot swab taken from various surfaces in the surrounding of the broiler farms turned out to
    be positive for ESBL as well as 10 % of the exhaust air samples. In contrast, the so far investigated turkey farms
    show a significantly lower contamination of slurry samples and of the surfaces in the vicinity of the barns.
    Faecal and airborne emission of ESBL/AmpC-producing organisms from broiler barns and in a significant lower extend
    also from turkey barns seems to be a possible source for the spread of ESBL in the environment. Further analyses
    on that topic are necessary.