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Extended-spectrum beta-lactamases (ESBL) reduce dramatically the effectiveness of modern extended-spectrum
cephalosporins and monobactams. The aim of this study was to comparing investigate the occurrence of these microorganisms
in the air and on soil surfaces close to ESBL/AmpC positive broiler and turkey fattening farms including
the possible spread of these bacteria via the faecal rout and/or via exhaust stable air in the environment.
Materials and Methods
We investigated seven German broiler and turkey fattening farms, each, three times during one fattening period.
Therefore, slurry samples, exhaust air samples and various surfaces in the vicinity of the barns were investigated.
Additionally various samples inside the barns including samples of animals, environmental samples and air samples
were taken in parallel. For phenotypic detection of ESBL/AmpC-producing E. coli all taken samples were cultivated
on MacConkey-agar with 1μg/ml Cefotaxime. The occurrence of different ESBL/AmpC-genes was confirmed by PCR
and if necessary by sequencing.
Preliminary results show a wide spread of ESBL/AmpC-producing E. coli in pig and broiler farms as well as an emission
in their surroundings. All slurry samples originating from broilers were positive for ESBL/AmpC-producing E. coli.
Additionally 28.8 % of all boot swab taken from various surfaces in the surrounding of the broiler farms turned out to
be positive for ESBL as well as 10 % of the exhaust air samples. In contrast, the so far investigated turkey farms
show a significantly lower contamination of slurry samples and of the surfaces in the vicinity of the barns.
Faecal and airborne emission of ESBL/AmpC-producing organisms from broiler barns and in a significant lower extend
also from turkey barns seems to be a possible source for the spread of ESBL in the environment. Further analyses
on that topic are necessary.