Fachbereich Veterinärmedizin



    MSSA and MRSA co-colonization dynamics and clonal diversity in pigs (2015)

    Friese, Anika (WE 10)
    Rösler, Uwe (WE 10)
    Kraushaar, Britta
    Fetsch, Alexandra
    XVII ISAH Congress 2015
    Košice, Slovakia, 07. – 11.06.2015
    Proceeding of the XVII International Congress of the International Society for Animal Hygiene “Animal hygiene and welfare in livestock production – the first step to food hygiene” — University of Veterinary Medicine and Pharmacy in Košice (Hrsg.)
    Košice, Slovakia: Edition centrum and specialised literature shop of University of Veterinary Medicine and Pharmacy in Košice, 2015 — S. 86
    ISBN: 978-80-8077-462-2
    URL (Volltext): http://www.isah-soc.org/userfiles/downloads/proceedings/2015_Proceedings.pdf
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845

    Abstract / Zusammenfassung

    Methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) are
    colonizers of skin and mucosa. Studies about MSSA and MRSA in human patients showed a competition of these
    two microorganisms for colonization space in the anterior nares. Moreover, in humans one clone can be found rather
    than differing types of MSSA and MRSA. Concerning a potential control strategy, this could also be important for
    pigs. Therefore, the aim of this study was to investigate the colonization dynamics of both MSSA and MRSA in pigs
    over a longer time period and investigate their clonal diversity.
    Materials and Methods
    Eighteen pigs were sampled three times every ten weeks with a nasal swab. Additionally, environmental samples
    such as swabs of walls, toys, brushes, watering places and troughs were taken. All samples were investigated for
    MSSA and MRSA, respectively. Spa-typing was done with up to five MRSA and MSSA isolates, respectively, found
    per sample and time point. Of almost 400 MSSA and MRSA isolated, 62 isolates were further investigated by microarray.
    We found three pigs to be non-carriers and twelve were colonized with both MRSA and MSSA. In 14 out of 54 samples
    taken from all animals over all-time points MSSA were found, only. In comparison, nine samples were exclusively
    MRSA-positive. Spa-types of MSSA and MRSA were mostly different with CC398 associated spa-types within
    MRSA isolates, whereas CC9 associated spa-types predominate as MSSA in both, pigs and their environment.
    Moreover, strains of the same clonal lineage showed a high genetic identity despite their origin.
    The results do not support the hypothesis of a competitive colonization of MSSA and MRSA in the anterior nares of
    pigs. Rather we found a changing status. Hence, highly identic clones of MSSA and MRSA, respectively, are present
    in the anterior nares of pigs and their environment.