Fachbereich Veterinärmedizin



    Occurence of carbapenem resistant and carbapenemase producing enterobacteriaceae (CPE) isolated on german pig-fattening farms (2015)

    Dr. Roschanski, Nicole (WE 10)
    Dr. Friese, Anika (WE 10)
    Käsbohrer, Annemarie
    Kreienbrock, Lothar
    Dr. Rösler, Uwe (WE 10)
    XVII ISAH Congress 2015
    Košice, Slovakia, 07. – 11.06.2015
    Proceeding of the XVII International Congress of the International Society for Animal Hygiene “Animal hygiene and welfare in livestock production – the first step to food hygiene” — University of Veterinary Medicine and Pharmacy in Košice (Hrsg.)
    Košice, Slovakia: University of Veterinary Medicine and Pharmacy in Košice, 2015 — S. 82
    ISBN: 978-80-8077-462-2
    URL (Volltext): http://www.isah-soc.org/userfiles/downloads/proceedings/2015_Proceedings.pdf
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845

    Abstract / Zusammenfassung

    1. Introduction
    As carbapenems are mostly considered as drug of last choice for the treatment of serious infections in human, the
    increasing spread of carbapenem resistance among Enterobacteriaceae is quite alarming. During the last couple of
    years a wide variety of carbapenemases has been isolated from cases of human infections, but just a few studies
    have reported their occurrence in livestock and livestock associated surroundings. However, since recently Enterobacteriaceae
    carrying blaVIM-1 genes have been isolated in German animal husbandries (Fischer et al., 2012; 2013),
    the monitoring of CPE in livestock became a major topic within the European Union.

    2. Materials and Methods
    Within the here described study a collection of 238 pooled feces and boot swab samples, chosen from a crosssectional
    study including 58 pig-fattening farms throughout Germany was investigated. The bacteria were selected
    on MacConkey agar plates containing 0.125 μg/ml Meropenem. Enterobacteriaceae which were able to grow on
    these plates were further investigated by using different phenotypic- as well as genotypic approaches.

    3. Results
    Four Escherichia coli, two Enterobacter cloacae and one Proteus penneri, showing either resistance or reduced susceptibilities
    against carbapenems, were isolated from five different farms.
    Two of the E. coli strains, derived from one farm, contained the carbapenemase gene blaVIM-1. The remaining Enterobacteriaceae
    did not show the presence of such a resistance gene. In these cases other resistance mechanisms,
    leading to reduced carbapenem susceptibility, were detected.

    4. Conclusions
    Until now, CPEs within German pig-fattening farms show a low prevalence (1 out of 58 farms, 1.72%). Furthermore,
    non-CPEs showing increased carbapenem tolerance have been detected. These findings indicate that Carbapenem
    resistant Enterobacteriaceae might be present in animal husbandries. To prevent a further spread of these bacteria
    between farms and farm anima