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The primary aim of this study was to detect and genotype Campylobacter coli strains from humans over a period of one year from November 2002 to October 2003. Campylobacter spp. were isolated from patients with symptoms of enteric infection. Amplified fragment length polymorphism (AFLP) analysis was used to identify the genetic diversity of C. coli strains by cluster analysis. A total of 18.6% of all Campylobacter isolates were identified as C. coli. These data show, that C. coli is indeed of importance to human campylobacter infections. Heterogeneous patterns were detectable among the human C. coli pool by AFLP analysis suggesting different sources of infection. A continuous seasonal shift of genotypes was detectable.