Publikationsdatenbank

The signalling molecule Autoinducer-2 is not internalised in Campylobacter jejuni (2015)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Adler, L. (WE 8)

Alter, T. (WE 8)

Sharbati, S. (WE 3)

Gölz, G. (WE 8)

Quelle

Berliner und Münchener tierärztliche Wochenschrift; 128(3-4) — S. 111–116

ISSN: 0005-9366

Sprache

Englisch

Verweise

DOI: 10.2376/0005-9366-128-111

Pubmed: 25876270

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62550
lebensmittelhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Bacteria recognise and constantly adjust to changing situations by sensing environmental and self-produced signals. Autoinducer-2 (AI-2) is a signal molecule, found in many bacterial species and thus proposed to enable interspecies communication. Two classes of AI-2 receptors have been identified so far. One class of AI-2 receptors sense AI-2 by a two component signalling system. The other class, the LsrB family, internalises and phosphorylates AI-2. Campylobacter (C.) jejuni is known to produce AI-2, but no AI-2 receptor in C. jejuni has been found yet. Therefore, all research on AI-2 dependent phenotypes has been conducted with AI-2 synthase (luxS) mutants. This mutation also leads to a disruption of the activated methyl cycle. Most studies lack sufficient complementation resulting in not knowing whether phenotypes of luxS mutants depend on disrupted metabolism or lack of AI-2. All this contributes to an intensive discussion, about the exact role of AI-2 in C. jejuni. Our previous study showed altering phenotypes (growth and motility) of the C. jejuni NCTC 11168ΔluxS mutant in contrast to wild type, which could be complemented with synthetic AI-2. To assess how AI-2 induces the altering phenotypes during complementation assays, we performed an AI-2 uptake assay. Our data show, that AI-2 is not internalised by C. jejuni. These data suggest, that yet unknown AI-2 receptors are present on the cell surface of C. jejuni. Therefore further search of AI-2 receptors in C. jejuni should focus on two component signalling systems and not on transporter systems.