Publikationsdatenbank

Molecular biological studies on Mycobacterium avium-intracellulare complex isolated from slaughtered pigs and wildlife animals in Germany (2004)

Art

Hochschulschrift

Autor

Sirimalaisuwan, Anucha (WE 8)

Quelle

Berlin, 2004 — 93 Seiten

Sprache

Englisch

Verweise

URL (Volltext): https://refubium.fu-berlin.de/handle/fub188/13329

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

In a cross-sectional study multiple lymph nodes from 217 pigs, 174 from conventionally managed farms and 43 from an "eco" farm in different geographic areas of Germany slaughtered in four slaughterhouses were investigated for their mycobacterial burden. By carcass inspection 96 pigs were found to exhibit pathological lesions, 121 did not show macroscopic signs of mycobacterial infection. Lymph node specimens from different organs of all 217 pigs were investigated by Ziehl-Neelsen staining and cultivation. The species of isolates were identified by PCR. Staining showed sensitivity of 81,16% compared to culture of tissue on Löwenstein-Jensen or Stonebrink medium. From 32,05% of the conventionally managed pigs without pathological lesions mycobacteria could be isolated, compared to 60,46% of "eco" pigs. In 29.66% of the animals mycobacteria were found in multiple lymph nodes, in some cases also in muscle lymph nodes. In 83 out of 217 pigs M. avium subspecies hominissuis was isolated. High resolution genotyping using RFLP and PFGE showed a high degree of intrasubspecies differences, but no relationship between RFLP and PFGE clusters. PFGE of M. avium ssp. hominissuis isolates from pigs originating from different regions slaughtered in one of the slaughterhouses showed close clonal relationship to each other and to cattle isolates from the geographical area of the respective slaughterhouse found in the year 2000. This clonal group comprised about 45% of all M. avium ssp. hominissuis isolates, while RFLP yielded a very diffiuse dendrogramme. Therefore PFGE is considered to be the more suitable method for large scale epidemiological investigations for M. avium infections.