Fachbereich Veterinärmedizin



    Charakterisierung der proinflammatorischen und endotoxischen Aktivität von organischen Staubfraktionen aus Tierställen in Abhängigkeit ihres aerodynamischen Durchmessers (2014)

    Chahoud, Mohsen (WE 10)
    Berlin: Mensch und Buch Verlag, 2014 — IV, 144 Seiten
    ISBN: 978-3-86387-434-6
    URL (Volltext): http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000096230
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    Tel.+49 30 8385 1845 Fax.+49 30 83845 1863

    Abstract / Zusammenfassung

    Airborne exposure to inflammation-inducing substances from Bioaerosols in both human medicine and veterinary medicine is associated with a wide range of symptoms and lung impairments. Currently for the quantification of such airborne exposure to inflammationinducing substances the LAL-Test is basically used to determine endotoxins.
    In addition to LAL-Test, test systems are used, which depend on releasing of proinflammatoric mediators from different cell systems.
    In contrast to the LAL-Test, these tests detect not only endotoxins, but also other proinflammatoric active substances, such as Glucans. One of these test systems is for example the so called Human Whole Blood Test. In this test, effective proinflammatoric substances are incubated with whole blood, then the releasing interleukin 1ß from blood monocytes is quantified. In the present work, the LAL Test and the Human Whole Blood Test were compared for their quantification of inflammation-inducing substances in airborne dust fractions from animal houses. For this, the inhalable dust fraction, the respirable dust fraction, the PM10 dust fraction and PM1.0 dust fraction were collected with standard filtration methods in different animal houses. Then the concentration of endotoxins and proinflammatoric substances in the samples was determined by using the above mentioned test systems. Furthermore, the obtained data were used for assessing whether endotoxins and proinflammatoric active substances may accumulate in one of the investigated dust fractions. In all dust fractions that were investigated in this work, a significant correlation between the endotoxic activity through LAL-Test and the proinflammatoric activity through Whole Blood Test could be detected. Thus, summarized it can be concluded, even though LAL-Test detects only endotoxins, it is also suitable to describe the airborne exposure to inflammation-inducing substances in animal stables.
    This result should be based on the fact, that endotoxins represent quantitatively and qualitatively the strongest inflammationinducing component in the air of animal houses. Furthermore the results of here carried out investigations indicate, that there is no specific accumulation of endotoxins and proinflammatoric active substances in anyone of the investigated dust fractions. A comparison of the volume-related proinflammatoric and endotoxic activity of in this work investigated dust fractions indicates, that the major part of the endotoxic and proinflammatoric activity is in the inhalable dust fraction and associated with particles, which are already deposited in the upper regions of the respiratory tract. Dust reduction measures in animal stables which, for example the nebulizing of oil, are especially effective in removing larger particles from the airborne state, should also contribute to a significant reduction in the total exposure to airborne endotoxins and inflammation-causing substances by both work staff and animals.