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    Proteomics analysis of ‘Orphan Species' - the case of Prototheca zopfii (family Chlorellaceae), the plant-like infectious organism:
    Proteomics analysis of ‘Orphan Species' - the case of Prototheca zopfii (family Chlorellaceae), the only known plant-like infectious organism (2014)

    Art
    Vortrag
    Autoren
    Murugaiyan, Jayaseelan (WE 10)
    Weise, Christoph
    Roesler, Uwe (WE 10)
    Kongress
    1st INPPO World Congress on Plant Proteomics: Methodology to Biology
    31.08. – 04.09.2014
    Quelle
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    Tel.+49 30 8385 1845 Fax.+49 30 83845 1863
    email:tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Proteomics analysis of unsequenced organisms (orphan species) is rare. The mass spectrometry-based protein identification of these species results in matching with protein sequences of other species and lower identification scores. One such species is Prototheca zopfii, an achlorophyllous alga associated with therapy-resistant bovine mastitis and rare human infection. Two genotypes of Prototheca zopfii have been described: non-pathogenic genotype 1 and pathogenic genotype 2. Recently, proteomic differences between these two genotypes were investigated using 2D-DIGE MALDI-TOF MS analysis (1) and NCBI database resulted in identification of 110 out of 209 proteins spots. Despite 280 Prototheca proteins entries (P. wickerhamii -272, P. zopfii -2, P. blaschkeae-1 and deduced from microbes-3) in the database, the rate of identification was low (52%) and the matching occurred with proteins from Prototheca (4%), other algae (36%), bacteria (39%), plants (10%), fungi (4%), and others (7%). Expressed sequence tag (EST)-based protein identification might enhance identification rate due to the larger number of EST entries (5906) in the database.
    1. Murugaiyan et al, 2013, Proteomics. 13, 2664-9