Fachbereich Veterinärmedizin



    Equine herpesvirus type 1 pUL56 modulates innate responses of airway epithelial cells (2014)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Soboll Hussey, Gisela
    Ashton, Laura V
    Quintana, Ayshea M
    Van de Walle, Gerlinde R
    Osterrieder, Nikolaus (WE 5)
    Lunn, David P
    Virology; 464-465 — S. 76–86
    ISSN: 0042-6822
    DOI: 10.1016/j.virol.2014.05.023
    Pubmed: 25046270
    Institut für Virologie

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    Abstract / Zusammenfassung

    Recently, the product of equine herpesvirus type 1 (EHV-1) ORF1, a homolog to HSV-1 pUL56, was shown to modulate MHC-I expression and innate immunity. Here, we investigated modulation of respiratory epithelial immunity by EHV-1 pUL56 and compared responses to those of PBMCs, which are important target cells that allow cell-associated EHV-1 viremia. The salient observations are as follows: (i) EHV-1 significantly down-modulated MHC-I and MHC-II expression in equine respiratory epithelial cells (ERECs). MHC-I expression remained unaffected in PBMCs and MHC-II expression was increased. (ii) Infection with an EHV-1 ORF1 deletion mutant partially restored MHC-I and MHC-II expression and altered IFN-alpha and IL-10 mRNA expression. (iii) Deletion of EHV-1 ORF1 also significantly increased chemokine expression and chemotaxis of monocytes and neutrophils in ERECs. Collectively, these results suggest a role for EHV-1 pUL56 in modulation of antigen presentation, cytokine expression and chemotaxis at the respiratory epithelium, but not in PBMC.