Fachbereich Veterinärmedizin



    Polar bear encephalitis:
    establishment of a comprehensive next-generation pathogen analysis pipeline for captive and free-living wildlife (2014)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Szentiks, C A
    Tsangaras, K
    Abendroth, B
    Scheuch, M
    Stenglein, M D
    Wohlsein, P
    Heeger, F
    Höveler, R
    Chen, W
    Sun, W
    Damiani, A (WE 5)
    Nikolin, V (WE 5)
    Gruber, A D
    Grobbel, M
    Kalthoff, D
    Höper, D
    Czirják, G Á
    Derisi, J
    Mazzoni, C J
    Schüle, A
    Aue, A
    East, M L
    Hofer, H
    Beer, M
    Osterrieder, N (WE 5)
    Greenwood, A D
    Journal of Comparative Pathology; 150(4) — S. 474–488
    ISSN: 0021-9975
    DOI: 10.1016/j.jcpa.2013.12.005
    Pubmed: 24534459
    Institut für Virologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51833

    Abstract / Zusammenfassung

    This report describes three possibly related incidences of encephalitis, two of them lethal, in captive polar bears (Ursus maritimus). Standard diagnostic methods failed to identify pathogens in any of these cases. A comprehensive, three-stage diagnostic 'pipeline' employing both standard serological methods and new DNA microarray and next generation sequencing-based diagnostics was developed, in part as a consequence of this initial failure. This pipeline approach illustrates the strengths, weaknesses and limitations of these tools in determining pathogen caused deaths in non-model organisms such as wildlife species and why the use of a limited number of diagnostic tools may fail to uncover important wildlife pathogens.