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    Zum impedimetrischen Nachweis von Antiinfektiva in Fleisch (1997)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Werber, D.
    Bergann, T. (WE 8)
    Quelle
    FleischWirtschaft : von der Erzeugung bis zur Vermarktung von Lebensmitteln tierischen Ursprungs; 77(06) — S. 556–559
    ISSN: 0015-363x
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    Tel.+49 30 838 62550 Fax.+49 30 838 46029
    email:lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Microbiological inhibition tests like the German Three-Plates-Test (TPT) or the New Netherland-Kidney-Test (NNKT) are requiring a relatively large amount of time until test results are available. Moreover theydo not seem to be appropriate for automation efforts. Using an impedimetric method could lead to certain improvements. In trials by using aMalthus Growth Analyzer, Bacillus stearothermophilus was found to be the most appropriate test organism. After investigations in order to find the appropriate framework, the sensitivity to detect certain antibiotics, i.e. Erythromycin, Gentamicin, Streptomycin, Chlortetracyclin,Oxytetracyclin, Penicillin G, Amoxicillin, Chloramphenicol and Polymyxin B, were examined and compared with the sensitivity of TPT and NNKT. Reliable results were available after an incubation period of 3.5 h. The sensitivity were within the maximum residue limits (MRLs) which were given by the European Community and seemed to be almost equivalentto those of the TPT. When using the impedimetric method, we suggest the use of meat liquid which can be gained by centrifugation.