Publikationsdatenbank

Putative connection between zoonotic multiresistant extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in dog feces from a veterinary campus and clinical isolates from dogs (2015)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Schaufler, Katharina (WE 7)

Bethe, Astrid (WE 7)

Lübke-Becker, Antina (WE 7)

Ewers, Christa

Kohn, Barbara (WE 20)

Wieler, Lothar H (WE 7)

Guenther, Sebastian (WE 7)

Quelle

Infection ecology & epidemiology

Bandzählung: 5

Seiten: 25334

ISSN: 2000-8686

Sprache

Englisch

Verweise

DOI: 10.3402/iee.v5.25334

Pubmed: 25656467

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

To contribute to the understanding of multiresistant bacteria, a 'One Health' approach in estimating the rate of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and getting insights into the transmission from clinical settings to the surrounding environment was employed by collecting fecal samples of dogs in a public area. Isolates were compared to those from samples of diseased dogs from a nearby small-animal clinic.

One hundred fecal samples of dogs were collected on a single day in the public area of a veterinary faculty with a small-animal clinic and adjacent residential neighborhoods. All identified ESBL-producing strains were isolated by selective plating, genotypically analyzed by DNA microarray, polymerase chain reaction, sequence analysis, and pulsed-field gel electrophoresis and compared to 11 clinical ESBL/AmpC-producing E. coli isolated from diseased dogs treated in the small-animal clinic 2 months before and 2 months following the environmental sampling collection.

Fourteen percent (14/100) of the extra-clinical samples harbored phenotypic ESBL/putative AmpC-producing E. coli with additional resistances against other antimicrobials. One ESBL-strain displayed an identical macrorestriction pattern to one clinical, another one to three clinical clonal ESBL-producing strains. The genotypic ESBL-determinants (blaCTX-M-1 and blaCTX-M-15) and detection rates (10%) in dog feces collected outside of the small-animal clinic are comparable to the rates and ESBL-types in the healthy human population in Germany and to clinical and non-clinical samples of humans and companion animals in Europe. The occurrence of identical strains detected both outside and inside the clinical setting suggests a connection between the small-animal clinic and the surrounding environment. In conclusion, dog feces collected in proximity to veterinary facilities should be considered as a non-point infection source of zoonotic ESBL-producing E. coli for both animals and humans. The common sniffing behavior of dogs further urges hygienic measures on the part of dog-patient owners, who should be educated to remove their pet's feces immediately and effectively.