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    Next Generation Sequencing Analysis of Biofilms from Three Dogs with Postoperative Surgical Site Infection (2014)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    König, L. M. (WE 12)
    Klopfleisch, R. (WE 12)
    Höper, D.
    Gruber, A. D. (WE 12)
    Quelle
    International Scholarly Research Notices; 2014 — S. Art.-ID 282971
    ISSN: 2356-7872
    Sprache
    Englisch
    Verweise
    DOI: 10.1155/2014/282971
    Kontakt
    Institut für Tierpathologie

    Robert-von-Ostertag-Str. 15
    Gebäude 12
    14163 Berlin
    Tel.+49 30 838 62450 Fax.+49 30 838 62522

    Abstract / Zusammenfassung

    The composition of biofilms in chronic wound infections of dogs is unclear. In the present study, histologically identified biofilms attached to sutures in chronically infected wounds of three dogs were examined by next generation sequencing of total DNA extracted from formalin-fixed and paraffin-embedded tissue samples. The analysis identified an inhomogeneous bacterial composition in three tissues containing biofilms. Some of the identified bacterial families such as Staphylococci and Streptococci have been found before in biofilms associated with human and canine wounds but in this study were quantitatively in the minority. The majority of the reads classified as bacterial sequences had the highest identity with sequences belonging to the Porphyromonadaceae, Deinococcaceae, Methylococcaceae, Nocardiaceae, Alteromonadaceae, and Propionibacteriaceae and thus taxons of so far minor relevance in veterinary medicine.