Publikationsdatenbank

Next Generation Sequencing Analysis of Biofilms from Three Dogs with Postoperative Surgical Site Infection (2014)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

König, L. M. (WE 12)

Klopfleisch, R. (WE 12)

Höper, D.

Gruber, A. D. (WE 12)

Quelle

International Scholarly Research Notices

Bandzählung: 2014

Seiten: Art.-ID 282971

ISSN: 2356-7872

Sprache

Englisch

Verweise

DOI: 10.1155/2014/282971

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The composition of biofilms in chronic wound infections of dogs is unclear. In the present study, histologically identified biofilms attached to sutures in chronically infected wounds of three dogs were examined by next generation sequencing of total DNA extracted from formalin-fixed and paraffin-embedded tissue samples. The analysis identified an inhomogeneous bacterial composition in three tissues containing biofilms. Some of the identified bacterial families such as Staphylococci and Streptococci have been found before in biofilms associated with human and canine wounds but in this study were quantitatively in the minority. The majority of the reads classified as bacterial sequences had the highest identity with sequences belonging to the Porphyromonadaceae, Deinococcaceae, Methylococcaceae, Nocardiaceae, Alteromonadaceae, and Propionibacteriaceae and thus taxons of so far minor relevance in veterinary medicine.