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    Epigenetic effects of dietary zinc on the porcine ZIP4gene expression (2015)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Karweina, D.
    Martin, Lena (WE 4)
    Pieper, Robert (WE 4)
    Zentek, Jürgen (WE 4)
    Brockmann, Gudrun A
    Quelle
    Perspectives in Science; 3(1/4) — S. 18–20
    ISSN: 2213-0209
    Sprache
    Englisch
    Verweise
    DOI: 10.1016/j.pisc.2014.11.011
    Kontakt
    Institut für Tierernährung

    Königin-Luise-Str. 49
    Gebäude 8
    14195 Berlin
    Tel.+49 30 838 52256 Fax.+49 30 838-55938
    email:tierernaehrung@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Dietary zinc supplementation has been shown to improve piglets’ health. We examined, if the gut epithelial ZIP4 transporter is affected by the zinc concentration in the diet through epigenetic modifications of the ZIP4 gene. In an experiment with 30 piglets that were fed diets with 57 (LZn ), 164 (NZn) or 2425 (HZn) mg zinc/kg feed over until four weeks, we found a reduced expression of the gene in the gut epithelium with higher zinc concentration in the feed (P ≤ 0.008). The methylation status of two CpGs in exon 2 and intron 2 were decreased in the LZn compared to the NZn group (P ≤ 0.01). The increase of the methylation at another CpG in exon 2 led to a decrease of the ZIP4 mRNA amount (P < 0.05). The fact, that only one CpG had a significant effect on ZIP4 expression, led us to the assumption that methylation changes play a minor role for the transcriptional regulation of ZIP4.