Fachbereich Veterinärmedizin



    Development of a multiplex real-time PCR for the rapid detection of the predominant beta-lactamase genes CTX-M, SHV, TEM and CIT-type AmpCs in Enterobacteriaceae (2014)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Roschanski, Nicole (WE 10)
    Fischer, Jennie
    Guerra, Beatriz
    Roesler, Uwe (WE 10)
    Langzeit-Monitoring von ESBL-bildenden und Fluor-Chinolon-resistenten Enterobacteriaceen in Nutztierhaltungen und deren Umgebung
    PLoS one; 9(7) — S. e100956
    ISSN: 1932-6203
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000020755
    DOI: 10.1371/journal.pone.0100956
    Pubmed: 25033234
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    Abstract / Zusammenfassung

    Beta-lactamase resistant bacteria and especially ESBL producing Enterobacteriaceae are an increasing problem worldwide. For this reason a major interest in efficient and reliable methods for rapid screening of high sample numbers is recognizable. Therefore, a multiplex real-time PCR was developed to detect the predominant class A beta-lactamase genes blaCTX-M, blaSHV, blaTEM and CIT-type AmpCs in a one-step reaction. A set of 114 Enterobacteriaceae containing previously identified resistance gene subtypes and in addition 20 undefined animal and environmental isolates were used for the validation of this assay. To confirm the accessibility in variable settings, the real-time runs were performed analogous in two different laboratories using different real-time cyclers. The obtained results showed complete accordance between the real-time data and the predetermined genotypes. Even if sequence analyses are further necessary for a comprehensive characterization, this method was proofed to be reliable for rapid screening of high sample numbers and therefore could be an important tool for e. g. epidemiological purposes or support infection control measures.