Fachbereich Veterinärmedizin



    RNAi in Arthropods:
    Insight into the Machinery and Applications for Understanding the Pathogen-Vector Interface (2012)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Barnard, Annette-Christi
    Nijhof, Ard M (WE 13)
    Fick, Wilma
    Stutzer, Christian
    Maritz-Olivier, Christine
    Genes; 3(4) — S. 702–741
    ISSN: 2073-4425
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000021368
    DOI: 10.3390/genes3040702
    Pubmed: 24705082
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35, 22, 23
    14163 Berlin
    Tel.+49 30 838 62310 Fax.+49 30 838 62323

    Abstract / Zusammenfassung

    The availability of genome sequencing data in combination with knowledge of expressed genes via transcriptome and proteome data has greatly advanced our understanding of arthropod vectors of disease. Not only have we gained insight into vector biology, but also into their respective vector-pathogen interactions. By combining the strengths of postgenomic databases and reverse genetic approaches such as RNAi, the numbers of available drug and vaccine targets, as well as number of transgenes for subsequent transgenic or paratransgenic approaches, have expanded. These are now paving the way for in-field control strategies of vectors and their pathogens. Basic scientific questions, such as understanding the basic components of the vector RNAi machinery, is vital, as this allows for the transfer of basic RNAi machinery components into RNAi-deficient vectors, thereby expanding the genetic toolbox of these RNAi-deficient vectors and pathogens. In this review, we focus on the current knowledge of arthropod vector RNAi machinery and the impact of RNAi on understanding vector biology and vector-pathogen interactions for which vector genomic data is available on VectorBase.