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    Arcobacter butzleri induces a pro-inflammatory response in THP-1 derived macrophages and has limited ability for intracellular survival (2014)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Bruegge, Jennifer Zur (WE 3)
    Hanisch, Carlos (WE 3)
    Einspanier, Ralf (WE 3)
    Alter, Thomas (WE 8)
    Gölz, Greta (WE 8)
    Sharbati, Soroush (WE 3)
    Forschungsprojekt
    SFB 852-TP B04: Untersuchungen zum Einfluss von intestinalen Faktoren wie Zink oder Mikroorganismen auf regulierende mircoRNA und Charakterisierung d. nachgeschalteten Reaktionsmechanismen
    Quelle
    International journal of medical microbiology; 304(8) — S. 1209–1217
    ISSN: 1438-4221
    Sprache
    Englisch
    Verweise
    DOI: 10.1016/j.ijmm.2014.08.017
    Pubmed: 25245281
    Kontakt
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62225
    biochemie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Recent case reports have identified Arcobacter (A.) butzleri to be another emerging pathogen of the family Campylobacteraceae causing foodborne diseases. However, little is known about its interaction with the human immune system. As macrophages act as first defense against bacterial infections, we studied for the first time the impact of A. butzleri on human macrophages using THP-1 derived macrophages as an in vitro infection model. Our investigations considered the inflammatory response, intracellular survival and activation of caspases as potential virulence mechanisms employed by A. butzleri. Induction of IL-1α, IL-1ß, IL-6, IL-8, IL-12ß and TNFα demonstrated a pro-inflammatory response of infected macrophages towards A. butzleri. gentamycin protection assays revealed the ability of A. butzleri strains to survive and resist the hostile environment of phagocytic immune cells for up to 22h. Moreover, initial activation of intitiator- (CASP8) as well as effector caspases (CASP3/7) was observed without the onset of DNA damage, suggesting a potential counter regulation. Intriguingly, we recognized distinct strain specific differences in invasion and survival capabilities. This suggests the existence of isolate dependent phenotype variations and different virulence potentials as known for other intestinal pathogens such as Salmonella enterica ssp.