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    Modelluntersuchungen zur Impedanzwirksamkeit lebensmittelhygienisch bedeutsamer Bakterienarten (1998)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Schulenburg, Jörg
    Bergann, Theodor (WE 8)
    Quelle
    Journal of veterinary medicine = Zentralblatt für Veterinärmedizin : Series B, Infectious diseases, immunobiology, food hygiene, public health; 45(9) — S. 551–559
    ISSN: 0721-1856
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    +49 30 838 62550
    lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The impedance technique mostly meets today's requirements of microbiological rapid methods. At relatively high prime cost for the equipmentthe advantages are marked by low personnel and material costs as wellas swiftness combined with highly flexible usage. The method is applicable for both quantitative and qualitative examinations but can fail occasionally in total count determination, especially if the sample material contains heterogeneous microbes. In model investigations with 53 strains of 17 different genera Enterobacteriaceae strains, Aeromonads and Enterococcus strains proved to be highly impedance effective. Lactobacillus strains and Pseudomonads as well as Staphylococcus aureus strains showed a low impedance effectiveness. Several strains, for example of the genera Micrococcus, Acinetobacter and Brochothrix, did notshow any changes of the medium impedance under the chosen conditions. Criterion for characterization of impedance effectiveness was the impedance detection time starting with identical initial counts (10(3) cfu/ml). Impedance effectiveness of microbes was determined at highly varying degree by the parameters of generation time, lag-phase duration and relative activity. This can lead either to wrong negative (underestimations) or wrong positive (overestimations) results of bacterial count.