Fachbereich Veterinärmedizin



    Microglial activation milieu controls regulatory T cell responses (2013)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Ebner, Friederike (WE 6)
    Brandt, Christine
    Thiele, Peggy
    Richter, Daniel
    Schliesser, Ulrike
    Siffrin, Volker
    Schueler, Jutta
    Stubbe, Tobias
    Ellinghaus, Agnes
    Meisel, Christian
    Sawitzki, Birgit
    Nitsch, Robert
    Journal of immunology; 191(11) — S. 5594–5602
    ISSN: 0022-1767
    DOI: 10.4049/jimmunol.1203331
    Pubmed: 24146044
    Institut für Immunologie und Molekularbiologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
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    Abstract / Zusammenfassung

    Although mechanisms leading to brain-specific inflammation and T cell activation have been widely investigated, regulatory mechanisms of local innate immune cells in the brain are only poorly understood. In this study, to our knowledge we show for the first time that MHC class II(+)CD40(dim)CD86(dim)IL-10(+) microglia are potent inducers of Ag-specific CD4(+)Foxp3(+) regulatory T cells (Tregs) in vitro. Microglia differentially regulated MHC class II expression, costimulatory molecules, and IL-10 depending on the amount of IFN-γ challenge and Ag dose, promoting either effector T cell or Treg induction. Microglia-induced Tregs were functionally active in vitro by inhibiting Ag-specific proliferation of effector T cells, and in vivo by attenuating experimental autoimmune encephalomyelitis disease course after adoptive transfer. These results indicate that MHC class II(+)CD40(dim)CD86(dim)IL-10(+) microglia have regulatory properties potentially influencing local immune responses in the CNS.