Fachbereich Veterinärmedizin



    Phylogenetic analysis of Staphylococcus aureus CC398 reveals a sub-lineage epidemiologically associated with infections in horses (2014)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Abdelbary, Mohamed M H
    Wittenberg, Anne
    Cuny, Christiane
    Layer, Franziska
    Kurt, Kevin
    Wieler, Lothar H (WE 7)
    Walther, Birgit (WE 7)
    Skov, Robert
    Larsen, Jesper
    Hasman, Henrik
    Fitzgerald, J Ross
    Smith, Tara C
    Wagenaar, J A
    Pantosti, Annalisa
    Hallin, Marie
    Struelens, Marc J
    Edwards, Giles
    Böse, R
    Nübel, Ulrich
    Witte, Wolfgang
    PLoS one; 9(2) — S. e88083
    ISSN: 1932-6203
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000021361
    DOI: 10.1371/journal.pone.0088083
    Pubmed: 24505386
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51840 / 51843

    Abstract / Zusammenfassung

    In the early 2000s, a particular MRSA clonal complex (CC398) was found mainly in pigs and pig farmers in Europe. Since then, CC398 has been detected among a wide variety of animal species worldwide. We investigated the population structure of CC398 through mutation discovery at 97 genetic housekeeping loci, which are distributed along the CC398 chromosome within 195 CC398 isolates, collected from various countries and host species, including humans. Most of the isolates in this collection were received from collaborating microbiologists, who had preserved them over years. We discovered 96 bi-allelic polymorphisms, and phylogenetic analyses revealed that an epidemic sub-clone within CC398 (dubbed 'clade (C)') has spread within and between equine hospitals, where it causes nosocomial infections in horses and colonises the personnel. While clade (C) was strongly associated with S. aureus from horses in veterinary-care settings (p = 2 × 10(-7)), it remained extremely rare among S. aureus isolates from human infections.