Publication Database

Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes (2009)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

von Samson-Himmelstjerna, Georg (WE 13)

Coles, Gerald C

Jackson, Frank

Bauer, Christian

Borgsteede, Fred

Cirak, Veli Y

Demeler, Janina (WE 13)

Donnan, Alison

Dorny, Pierre

Epe, Christian

Harder, Achim

Höglund, Johan

Kaminsky, Ronald

Kerboeuf, Dominique

Küttler, Ulla

Papadopoulos, Elias

Posedi, Janez

Small, John

Várady, Marián

Vercruysse, Jozef

Wirtherle, Nicole

Quelle

Parasitology research

Bandzählung: 105

Heftzählung: 3

Seiten: 825 – 834

ISSN: 0932-0113

Sprache

Englisch

Verweise

DOI: 10.1007/s00436-009-1466-1

Pubmed: 19452165

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The ability to reliably detect anthelmintic resistance is a crucial part of resistance management. If data between countries are to be compared, the same test should give the same results in each laboratory. As the egg hatch test for benzimidazole resistance is used for both research and surveys, the ability of different laboratories to obtain similar results was studied through testing of known isolates of cyathostomins, Haemonchus contortus, Ostertagia ostertagi, and Cooperia oncophora in programs supported by the EU (Cost B16 and FP6-PARASOL). Initial results showed difficulties in obtaining reproducible and similar data within and between laboratories. A series of ring tests, i.e., simultaneous and coordinated rounds of testing of nematode isolates in different laboratories was subsequently performed. By adopting identical protocols, especially the use of deionized water and making dilutions of thiabendazole in dimethyl sulfoxide in the final ring test, laboratories correctly identified both susceptible and resistant isolates. The protocols for the test and preparation of solutions of thiabendazole are described.