Fachbereich Veterinärmedizin



    Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes (2009)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    von Samson-Himmelstjerna, Georg (WE 13)
    Coles, Gerald C
    Jackson, Frank
    Bauer, Christian
    Borgsteede, Fred
    Cirak, Veli Y
    Demeler, Janina (WE 13)
    Donnan, Alison
    Dorny, Pierre
    Epe, Christian
    Harder, Achim
    Höglund, Johan
    Kaminsky, Ronald
    Kerboeuf, Dominique
    Küttler, Ulla
    Papadopoulos, Elias
    Posedi, Janez
    Small, John
    Várady, Marián
    Vercruysse, Jozef
    Wirtherle, Nicole
    Parasitology research; 105(3) — S. 825–834
    ISSN: 0932-0113
    DOI: 10.1007/s00436-009-1466-1
    Pubmed: 19452165
    Institut für Parasitologie und Tropenveterinärmedizin

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35, 22, 23
    14163 Berlin
    +49 30 838 62310

    Abstract / Zusammenfassung

    The ability to reliably detect anthelmintic resistance is a crucial part of resistance management. If data between countries are to be compared, the same test should give the same results in each laboratory. As the egg hatch test for benzimidazole resistance is used for both research and surveys, the ability of different laboratories to obtain similar results was studied through testing of known isolates of cyathostomins, Haemonchus contortus, Ostertagia ostertagi, and Cooperia oncophora in programs supported by the EU (Cost B16 and FP6-PARASOL). Initial results showed difficulties in obtaining reproducible and similar data within and between laboratories. A series of ring tests, i.e., simultaneous and coordinated rounds of testing of nematode isolates in different laboratories was subsequently performed. By adopting identical protocols, especially the use of deionized water and making dilutions of thiabendazole in dimethyl sulfoxide in the final ring test, laboratories correctly identified both susceptible and resistant isolates. The protocols for the test and preparation of solutions of thiabendazole are described.