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    A novel high-resolution melt PCR assay discriminates Anaplasma phagocytophilum and "Candidatus Neoehrlichia mikurensis" (2013)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Krücken, Jürgen (WE 13)
    Schreiber, Cécile
    Maaz, Denny (WE 13)
    Kohn, Mareen
    Demeler, Janina (WE 13)
    Beck, Stefanie
    Schein, Eberhard
    Olias, Philipp
    Richter, Dania
    Matuschka, Franz-Rainer
    Pachnicke, Stefan
    Krieger, Klemens
    Kohn, Barbara (WE 20)
    von Samson-Himmelstjerna, Georg (WE 13)
    Quelle
    Journal of clinical microbiology; 51(6) — S. 1958–1961
    ISSN: 0095-1137
    Sprache
    Englisch
    Verweise
    DOI: 10.1128/JCM.00284-13
    Pubmed: 23576542
    Kontakt
    Klinik für kleine Haustiere

    Oertzenweg 19 b
    Haus 1
    14163 Berlin
    +49 30 838 62356
    kleintierklinik@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    "Candidatus Neoehrlichia mikurensis" (Anaplasmataceae) is an emerging pathogen transmitted by Ixodes ticks. Conventional PCR and the newly developed high-resolution melt PCR were used to detect and discriminate "Candidatus Neoehrlichia mikurensis" and Anaplasma phagocytophilum. Both bacterial species were frequently found in Ixodes ricinus and Ixodes hexagonus but virtually absent from Dermacentor reticulatus. In rodents, "Candidatus N. mikurensis" was significantly more prevalent than A. phagocytophilum, whereas in cats, only A. phagocytophilum was found.