Publikationsdatenbank

A novel high-resolution melt PCR assay discriminates Anaplasma phagocytophilum and "Candidatus Neoehrlichia mikurensis" (2013)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Krücken, Jürgen (WE 13)

Schreiber, Cécile

Maaz, Denny (WE 13)

Kohn, Mareen

Demeler, Janina (WE 13)

Beck, Stefanie

Schein, Eberhard

Olias, Philipp

Richter, Dania

Matuschka, Franz-Rainer

Pachnicke, Stefan

Krieger, Klemens

Kohn, Barbara (WE 20)

von Samson-Himmelstjerna, Georg (WE 13)

Quelle

Journal of clinical microbiology

Bandzählung: 51

Heftzählung: 6

Seiten: 1958 – 1961

ISSN: 0095-1137

Sprache

Englisch

Verweise

DOI: 10.1128/JCM.00284-13

Pubmed: 23576542

Kontakt

Klein- und Heimtierklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62422
kleintierklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

"Candidatus Neoehrlichia mikurensis" (Anaplasmataceae) is an emerging pathogen transmitted by Ixodes ticks. Conventional PCR and the newly developed high-resolution melt PCR were used to detect and discriminate "Candidatus Neoehrlichia mikurensis" and Anaplasma phagocytophilum. Both bacterial species were frequently found in Ixodes ricinus and Ixodes hexagonus but virtually absent from Dermacentor reticulatus. In rodents, "Candidatus N. mikurensis" was significantly more prevalent than A. phagocytophilum, whereas in cats, only A. phagocytophilum was found.