Fachbereich Veterinärmedizin


Service-Navigation

    Publikationsdatenbank

    Detection of integrated herpesvirus genomes by fluorescence in situ hybridization (FISH) (2013)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autor
    Kaufer, Benedikt B (WE 5)
    Quelle
    Methods in molecular biology; 1064 — S. 141–152
    ISSN: 1064-3745
    Sprache
    Englisch
    Verweise
    DOI: 10.1007/978-1-62703-601-6_10
    Pubmed: 23996255
    Kontakt
    Institut für Virologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51833
    viro@zedat.fu-berlin.de

    Abstract / Zusammenfassung

    Fluorescence in situ hybridization (FISH) is widely used to visualize nucleotide sequences in interphase cells or on metaphase chromosomes using specific probes that are complementary to the respective targets. Besides its broad application in cytogenetics and cancer research, FISH facilitates the localization of virus genomes in infected cells. Some herpesviruses, including human herpesvirus 6 (HHV-6) and Marek's disease virus (MDV), have been shown to integrate their genetic material into host chromosomes, which allows transmission of HHV-6 via the germ line and is required for efficient MDV-induced tumor formation. We describe here the detection by FISH of integrated herpesvirus genomes in metaphase chromosomes and interphase nuclei of herpesvirus-infected cells.