Publikationsdatenbank

Glycoprotein H and α4β1 integrins determine the entry pathway of alphaherpesviruses (2013)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Azab, Walid (WE 5)

Lehmann, Maik J

Osterrieder, Nikolaus (WE 5)

Quelle

Journal of virology : publ. by the American Society for Microbiology

Bandzählung: 87

Heftzählung: 10

Seiten: 5937 – 5948

ISSN: 1098-5514

Sprache

Englisch

Verweise

DOI: 10.1128/JVI.03522-12

Pubmed: 23514881

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Herpesviruses enter cells either by direct fusion at the plasma membrane or from within endosomes, depending on the cell type and receptor(s). We investigated two closely related herpesviruses of horses, equine herpesvirus type 1 (EHV-1) and EHV-4, for which the cellular and viral determinants routing virus entry are unknown. We show that EHV-1 enters equine epithelial cells via direct fusion at the plasma membrane, while EHV-4 does so via an endocytic pathway, which is dependent on dynamin II, cholesterol, caveolin 1, and tyrosine kinase activity. Exchange of glycoprotein H (gH) between EHV-1 and EHV-4 resulted in rerouting of EHV-1 to the endocytic pathway, as did blocking of α4β1 integrins on the cell surface. Furthermore, a point mutation in the SDI integrin-binding motif of EHV-1 gH also directed EHV-1 to the endocytic pathway. Cumulatively, we show that viral gH and cellular α4β1 integrins are important determinants in the choice of alphaherpesvirus cellular entry pathways.