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    Strain Background, Kind of Mutation And Experimental Conditions Impact the Phenotypes of "Campylobacter jejuni" "luxS" Mutants (2014)

    Art
    Poster
    Autoren
    Adler, L. (WE 8)
    Alter, T. (WE 8)
    Sharbati, S. (WE 3)
    Gölz, G. (WE 8)
    Kongress
    American Society For Microbiology, 114th General Meeting
    Boston, Massachusetts, 17. – 20.05.2014
    Quelle
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://www.asmonlineeducation.com/php/asm2014abstracts/data/index.htm
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    +49 30 838 62550
    lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Background: Since the discovery that Campylobacter (C.) jejuni produces Autoinducer 2 (AI-2), various studies have been conducted to explore the function and role of AI-2 in C. jejuni. However, the interpretation of these analyses has been complicated by differences in strain backgrounds, kind of mutation and culture conditions used. Furthermore, the research on AI-2 dependent phenotypes has to be conducted with AI-2 synthase (luxS) mutants as no AI-2 receptor has been found yet. But the mutation of luxS also leads to a disruption of the activated methyl cycle. Most studies lack sufficient complementation resulting in not knowing whether phenotypes of luxS mutants depend on disrupted metabolism or lack of AI-2. All this contributes to an intensive discussion about the exact role of AI-2 in C. jejuni.
    Materials: We examined the impact of different media, temperature and chemically complementation on growth and motility of three different C. jejuni luxS mutants. Results: Our study showed that growth and motility of C. jejuni luxS mutants depend on strain background, mutation strategy and culture conditions. Complementation with AI-2 and AI-2+homocysteine significantly increased the cell number of C. jejuni NCTC 11168αluxS in stationary phase compared to the non-complemented C. jejuni NCTC 11168αluxS mutant. Also swarming ability could be partially complemented. With neither complementation, the phenotype of the wild type-strains was achieved, suggesting yet another reason for differing phenotypes other than communication and methionine metabolism for C. jejuni luxS mutants.
    Conclusion: Further research should focus on the search for the AI-2 receptor to elucidate the exact role of AI-2 dependent behaviour in C. jejuni.