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    Campylobacter infection in chickens modulates the intestinal epithelial barrier function (2015)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Awad, W. A.
    Molnár, A.
    Aschenbach, Jörg R. (WE 2)
    Ghareeb, K.
    Khayal, B.
    Hess, C.
    Liebhart, D.
    Dublecz, K.
    Hess, M.
    Quelle
    Innate immunity; 21(2) — S. 151–160
    ISSN: 1753-4259
    Sprache
    Englisch
    Verweise
    DOI: 10.1177/1753425914521648
    Pubmed: 24553586
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    Institut für Veterinär-Physiologie

    Oertzenweg 19 b
    14163 Berlin
    Tel.+49 30 838 62600 Fax.+49 30 838-62610
    email:physiologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Asymptomatic carriage of Campylobacter jejuni is highly prevalent in chicken flocks. Thus, we investigated whether chronic Campylobacter carriage affects chicken intestinal functions despite the absence of clinical symptoms. An experiment was carried out in which commercial chickens were orally infected with C. jejuni (1 × 108 CFU/bird) at 14 days of life. Changes in ion transport and barrier function were assessed by short-circuit current (Isc) and transepithelial ion conductance (Gt) in Ussing chambers. Gt increased in cecum and colon of Campylobacter-infected chicken 7 d post-infection (DPI), whereas Gt initially decreased in the jejunum at 7 DPI and increased thereafter at 14 DPI. The net charge transfer across the epithelium was reduced or tended to be reduced in all segments, as evidenced by a decreased Isc. Furthermore, the infection induced intestinal histomorphological changes, most prominently including a decrease in villus height, crypt depth and villus surface area in the jejunum at 7 DPI. Furthermore, body mass gain was decreased by Campylobacter carriage. This study demonstrates, for the first time, changes in the intestinal barrier function in Campylobacter-infected chickens and these changes were associated with a decrease in growth performance in otherwise healthy-appearing birds.