Publikationsdatenbank

Further analysis of anti-human leukocyte mAbs with reactivity to equine leukocytes by two-colour flow cytometry and immunohistochemistry (2007)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Flaminio, B F

Ibrahim, Sherif

Lunn, D Paul

Stark, Robert

Steinbach, Falko

Quelle

Veterinary immunology and immunopathology

Bandzählung: 119

Heftzählung: 1-2

Seiten: 92 – 9

ISSN: 0165-2427

Sprache

Englisch

Verweise

Pubmed: 17706294

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

We have reported on the reactivity of anti-human CD molecules with equine leukocytes by single-colour flow cytometry (this issue). The objectives of this additional study were to test for the reliability of the results obtained, and to obtain further information on the positive populations of lymphocytes. Two-colour flow cytometry and immunohistochemistry were performed, using many of the positive mAbs and a few questionable ones from the first part of the study. All mAbs analysed by two-colour flow cytometry could be confirmed to their previous designation as "positive" or "questionable". Most of the mAbs tested were effective in immunohistochemistry, supporting previous results. Examples of positive results will be presented and limitations of the study will be discussed briefly.