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Campylobacter spp is a major cause of gastroenteritis throughout the world, in underdeveloped and developed countries alike. Oral contraction is the primary route of infection chiefly occurring by consumption of contaminated water and undercooked meat products. Because of selective pressures, such as the environment, antibiotic usage and carrier species, Campylobacter spp has undergone evolution. In the present study, the genotypic diversity and dynamics of Campylobacter coli were invested by means of MSLT, a molecular method of detection which allows for highly sensitive, specific and rapid identification of Campylobacter sequencing types. By using MSLT and comparing the isolates from different areas of the world and from different species of origin, some hypothesis can be formed about the route of transmission and the evolution of Campylobacter coli.